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KMID : 0950619960050010061
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Journanl of Allied Health Sciences
1996 Volume.5 No. 1 p.61 ~ p.70
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Detection of Methicillin-Resistant Staphylococcus aureus by Multiplex PCR
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Lee Seung-Gwan
Lee Chang-Kyu
Lee Dong-Ho
Whang Sun-Chul
Yoon Hyo-Sook
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Abstract
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In Staphytococcus aureus, mecA and nuc genes are the genetic determinants of methicillin resistance and thermostable nuclease. Using a multiplex PCR strategy. 533- and 267-bp regions of mecA and nuc genes, respectively, were coamplified to identify susceptible(lacking mecA) and resistant (mecA) Staphylococci and to differentiate S. aureus(nuc^(+)) from coagulase negative Staphylococci(lacking nuc). Two hundred seven Staphylococcal strains were tested. All 125 methidllin-resistant strains were found to be mecA^(+), and 33 of the 34 susceptible isolated lacked mecA. 37 methicillin resistant coagulase-negative Staphylococci(CNS) were also found to be mecA^(+), while 11 susceptible isolates lacked mecA. The nuc determinant was a unique feature of S. aureus ´, it was found in 100% of the S. aureus strains tested but was not detected in all the coagulase-negative Staphylococci(CNS). In conclusion, coamplification of mecA and nuc determinants proved to be very reliable both for the rapid detection of methicillin resistance and differential diagnosis between S. aureus and other Staphylococci. This technique could be a useful tool not only in the diagnosis and classification of drug resistant pathogenic microorganisms but also for the monitoring the strategy of treatment for staphylococcal infections.
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